Effect of Ozone on Bacteria

  • Computer generated image of a bacteria cell
  • Close-up of ozone molecule coming into contact with bacterial wall
  • Ozone penetrating and creating hole in bacterial wall
  • Close-up effect of ozone on cell wall
  • Bacterial cell after a few ozone molecules come into contact
  • Destruction of cell after ozone (cell lysing)

Ozone Effects on Specific Bacteria, Viruses and Molds

Bacteria are microscopically small, single-cell creatures having a primitive structure. The bacteria body is sealed by a relatively solid-cell membrane. Ozone interferes with the metabolism of bacterium-cells, most likely through inhibiting and blocking the operation of the enzymatic control system. A sufficient amount of ozone breaks through the cell membrane, and this leads to the destruction of the bacteria.

Viruses are small, independent particles, built of crystals and macromolecules, Unlike bacteria, they multiply only within the host cell. They transform protein of the host cell into proteins of their own. Ozone destroys viruses by diffusing through the protein coat into the nucleic acid core, resulting in damage of the viral RNA. At higher concentrations, ozone destroys the capsid, or exterior protein shell by oxidation so DNA (deoxyribonucleic acid), or RNA (ribonucleic acid) structures of the microorganism are affected.


Raw Water Disinfection

Portable Water Disinfection


1-mg/l = 1-PPM

Pathogen

Dosage

Aspergillus Niger (Black Mount)

Destroyed by 1.5 to 2 mg/I

Bacillus Bacteria

Destroyed by 0.2 m/I within 30 seconds 

Bacillus Anthracis (causes anthrax in sheep, cattle and pigs.  Also a human pathogen)

Ozone susceptible

Bacillus cereus

99% destruction after 5-min at 0.12 mg/l in water

B. cereus (spores)

99% destruction after 5-min at 2.3 mg/l in water

Bacillus subtilis

90% reduction at 0.10-PPM for 33 minutes

Bacteriophage f2

99.99% destruction at 0.41 mg/l for 10-seconds in water

Botrytis cinerea

3.8 mg/l for 2 minutes

c. difficile

99.999% destruction at 0.6 mg/l for 2 minutes (in water)

Candida Bacteria

Ozone susceptible

Clavibacter michiganense

99.99% destruction at 1.1 mg/l for 5 minutes

Cladosporium

90% reduction at 0.10-PPM for 12.1 minutes

Clostridium Bacteria

Ozone susceptible

Clostridium Botulinum Spores. Its toxin paralyses the central nerve system, being a poison multiplying in food and meals.

0.4 to 0.5 mg/l threshold value

Coxsackie Virus A9

95% destruction at 0.035 mg/l for 10-seconds in water 

Coxsackie Virus B5

99.99% destruction at 0.4 mg/l for 2.5-minutes in sludge effluent 

Diphtheria Pathogen

Destroyed by 1.5 to 2 mg/l

Eberth Bacillus (Typhus abdomanalis).  Spreads typically by aqueous infection and causes typhoid.

Destroyed by 1.5 to 2 mg/l

Echo Virus 29: The virus most sensitive to ozone.

After a contact time of 1 minute at 1 mg/l of ozone, 99.999% killed. 

Enteric virus

95% destruction at 4.1 mg/l for 29 minutes in raw wastewater

Escherichia Coli Bacteria (from feces) 

Destroyed by 0.2 mg/l within 30 seconds in air

E-coli (in clean water)

99.99% destruction at 0.25 mg/l for 1.6 minutes

E-coli (in wastewater)

99.9% destruction at 2.2 mg/l for 19 minutes

Encephalomyocarditis Virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l.

Endamoebic Cysts Bacteria

Ozone susceptible

Enterovirus Virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l.

Fusarium oxysporum f.sp. lycopersici

1.1 mg/l for 10 minutes

Fusarium oxysporum f.sp. melonogea

99.99 % destruction at 1.1 mg/l for 20 minutes

GDVII Virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l.

Hepatitis A virus

99.5% reduction at 0.25 mg/l for 2-seconds in a phosphate buffer

Herpes Virus

Destroyed to zero level in less than 30 seconds wit 0.1 to 0.8 mg/l.

Influenza Virus

0.4 to 0.5 mg/l threshold value

Klebs-Loffler Bacillus

Destroyed by 1.5 to 2 mg/l

Legionella pneumophila

99.99% destruction at 0.32 mg/l for 20 minutes in distilled water

Luminescent Basidiomycetes (species having no melanin pigment).

Destroyed in 10 minutes at 100-PPM

Mucor piriformis

3.8 mg/l for 2 minutes

Mycobacterium avium

99.9% with a CT value of 0.17 in water
(scientifically reviewed document)

Mycobacterium foruitum

90% destruction at 0.25 mg/l for 1.6 minutes in water

Penicillium Bacteria

Ozone susceptible

Phytophthora parasitica

3.8 mg/l for 2 minutes

Poliomyelitis Virus

99.99% kill with 0.3 to 0.4 mg/l in 3-4 minutes

Poliovirus type 1

99.5% destruction at 0.25 mg/l for 1.6 minutes in water

Proteus Bacteria

Very susceptible

Pseudomonas Bacteria

Very susceptible

Rhabdovirus virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l

Salmonella Bacteria

Very susceptible

Salmonella typhimurium

99.99% destruction at 0.25 mg/l for 1.67 minutes in water

Schistosoma Bacteria

Very susceptible

Staph epidermidis

90% reduction at 0.1-ppm for 1.7 min

Staphylococci

Destroyed by 1.5 to 2.0 mg/l

Stomatitis Virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l

Streptococcus Bacteria

Destroyed by 0.2 mg/l within 30 seconds

Verticillium dahliae

99.99 % destruction at 1.1 mg/l for 20 minutes

Vesicular Virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l

Virbrio Cholera Bacteria

Very susceptible

Vicia Faba progeny

Ozone causes chromosome aberration and its effect is twice that observed by the action of X-rays



The effect of ozone below a certain critical concentration value is small or zero. Above this level all pathogens are eventually destroyed. This effect is called all-or-none response and the critical level the "threshold value”.

Pathogen

Dosage

Vesicular Virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l

Streptococcus Bacteria

Destroyed by 0.2 mg/l within 30 seconds

Stomatitis Virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l

Salmonella typhimurium

99.99% destruction at 0.25 mg/l for 1.67 minutes in water

Staph epidermidis

90% reduction at 0.1-ppm for 1.7 min

Poliomyelitis Virus

99.99% kill with 0.3 to 0.4 mg/l in 3-4 minutes

Poliovirus type 1

99.5% destruction at 0.25 mg/l for 1.6 minutes in water

Proteus Bacteria

Very susceptible

Pseudomonas Bacteria

Very susceptible

Rhabdovirus virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l

Mycobacterium avium

99.9% with a CT value of 0.17 in water
(scientifically reviewed document)

Mycobacterium foruitum

90% destruction at 0.25 mg/l for 1.6 minutes in water

Penicillium Bacteria

Ozone susceptible

Legionella pneumophila

99.99% destruction at 0.32 mg/l for 20 minutes in distilled water

Legionella pneumophila

99.99% destruction at 0.32 mg/l for 20 minutes in distilled water

Luminescent Basidiomycetes (species having no melanin pigment).

Destroyed in 10 minutes at 100-PPM

GDVII Virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l.

Hepatitis A virus

99.5% reduction at 0.25 mg/l for 2-seconds in a phosphate buffer

Herpes Virus

Destroyed to zero level in less than 30 seconds wit 0.1 to 0.8 mg/l.

Encephalomyocarditis Virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l.

Endamoebic Cysts Bacteria

Ozone susceptible

Enterovirus Virus

Destroyed to zero level in less than 30 seconds with 0.1 to 0.8 mg/l.

Escherichia Coli Bacteria (from feces) 

Destroyed by 0.2 mg/l within 30 seconds in air

E-coli (in clean water)

99.99% destruction at 0.25 mg/l for 1.6 minutes